Chonroitinase ABC

Code: SAE0150-0.1ML D2-231

Application

Degradation of Chondroitin sulfate-containing tissue such as extracellular matrices and cartilage Neural regeneration research Eliminative degradation of all form...


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Your Price
€471.10 EACH
€579.45 inc. VAT

Application

Degradation of Chondroitin sulfate-containing tissue such as extracellular matrices and cartilage Neural regeneration research Eliminative degradation of all forms of Chondroitin sulfate: A, B and C Quantitation of glucosaminoglycans (mucopolysaccharides)

Compatibility

pH optimum: pH 8.0 (chondroitin sulfate) pH 6.8 (hyaluronic acid)Temperature optimum: 37 °CActivator: 0.05 M acetate Inhibitors: 1 mM Zn2+ Ni2+, Fe2+ and Cu2+ 

Components

Non active Ingredients: Hepes-Sodium salt, NaCl, Trehalose, Imidazole, Glycerol, Water

Features and Benefits

Low Endotoxin BSA Free Recombinant Endolytic component only, does not contain exolytic enzyme. Compatible with chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate, and acts slowly on hyaluronate

General description

Chondroitinase ABC is a lyase with specificity toward chondroitin sulfates and dermatan sulfates - with some minor activity toward hyaluronan. This recombinant version only contains the endolytic chondroitinase, meaning it will not digest the non-reducing ends of proteoglycan substrates. It targets β-(1,4)-linkages between N-acetylgalactosamine and either D-glucuronic acid or L-iduronic acid. The product of digestion is a mixture of δ 4-unsaturated tetra- and di-saccharides.

Other Notes

This product is for R&D use only. Not for drug, household, or other uses. Please consult the SafetyData Sheet for information regarding hazards and safe handling practices.

Storage and Stability

Store the product at -70 °C. When stored unopened at -70 °C, the buffer is stable for at least two years.

Unit Definition

1 unit will liberate 1.0 µmole of unsaturated disaccharides per min at pH 8.0 at 37 °C, using chondroitin sulfate from shark cartilage (C4384) as substrate.

biological sourcebacterial (Proteus vulgaris)
conjugate(Glucosaminoglycan)
Quality Level200
shipped indry ice
specific activity50-250 units/mg protein
storage temp.−70°C
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